Karsten Siller, PhD

Karsten Siller, PhD

User Services, Director

Karsten’s background is in developmental cell biology, genetics and light microscopy. He leads Research Computing’s User Services team and provides technical support, training and project consultation for UVA’s centralized compute platforms. His focus areas are HPC workflows, software containers, Python programming and image processing.

Selected Publications

  • Best MN, Lim Y, Ferenc NN, Kim N, Min L, Wang DB, Sharifi K, Wasserman AE, McTavish SA, Siller KH, Jones MK, Jenkins PM, Mandell JW, Bloom GS. Extracellular Tau Oligomers Damage the Axon Initial Segment. J Alzheimers Dis. 2023 May 9. doi:10.3233/JAD-221284.

  • Alam SR, Wallrabe H, Christopher KG, Siller KH, Periasamy A. Characterization of mitochondrial dysfunction due to laser damage by 2-photon FLIM microscopy. Sci Rep. 2022 Jul 13;12:11938.
    https://doi.org/10.1038/s41598-022-15639-z

  • Cao R, Wallrabe H, Siller K, Periasamy A. Optimization of FLIM imaging, fitting and analysis for auto-fluorescent NAD(P)H and FAD in cells and tissues. Methods Appl Fluoresc. 2020 Feb 5;8(2):024001. doi:10.1088/2050-6120/ab6f25.

  • Lauren K Rudenko LK, Wallrabe H, Periasamy A, Siller K, Svindrych Z, Seward ME, Best MN, Bloom GS. Intraneuronal Tau Misfolding Induced by Extracellular Amyloid-β Oligomers. J Alzheimers Dis. 2019;71(4):1125-1138. doi:10.3233/JAD-190226.

  • Cao R, Wallrabe H, Siller K, Rehman Alam S, Periasamy A. Single-cell redox states analyzed by fluorescence lifetime metrics and tryptophan FRET interaction with NAD(P)H.
    Cytometry A. 2019 Jan;95(1):110-121. doi:10.1002/cyto.a.23711.

  • Wallrabe H, Svindrych Z, Alam SR, Siller KH, Wang T, Kashatus D, Hu S, Periasamy A. Segmented cell analyses to measure redox states of autofluorescent NAD(P)H, FAD & Trp in cancer cells by FLIM. Sci Rep. 2018 Jan 8;8(1):79. doi:10.1038/s41598-017-18634-x.

  • Janssens DH, Hamm DC, Anhezini L, Xiao Q, Siller KH, Siegrist SE, Harrison MM, Lee CY. An Hdac1/Rpd3-Poised Circuit Balances Continual Self-Renewal and Rapid Restriction of Developmental Potential during Asymmetric Stem Cell Division. Dev Cell. 2017 Feb 27;40(4):367-380.e7. doi:10.1016/j.devcel.2017.01.014.

  • Doyle SE, Pahl MC, Siller KH, Ardiff L, Siegrist SE. Neuroblast niche position is controlled by Phosphoinositide 3-kinase-dependent DE-Cadherin adhesion. Development. 2017 Mar 1;144(5):820-829. doi:10.1242/dev.136713.

  • Siller KH, Doe CQ. Spindle orientation during asymmetric cell division. Nat Cell Biol. 2009 Apr;11(4):365-74. doi:10.1038/ncb0409-365.

  • Siller KH, Cabernard C, Doe CQ. The NuMA-related Mud protein binds Pins and regulates spindle orientation in Drosophila neuroblasts. Nat Cell Biol. 2006 Jun;8(6):594-600. doi:10.1038/ncb1412.

  • Siller KH, Serr M, Steward R, Hays TS, Doe CQ. Live imaging of Drosophila brain neuroblasts reveals a role for Lis1/dynactin in spindle assembly and mitotic checkpoint control. Mol Biol Cell. 2005 Nov;16(11):5127-40. doi:10.1091/mbc.e05-04-0338.

See Google Scholar for a complete list of publications.