/tag/pharmacology
Analysis of Calcium Activity in Ex Vivo Adrenal Slices
This proposal is to enhance the computational analysis of calcium imaging data from live adrenal tissue in the study of primary aldosteronism, a leading cause of hypertension. The significant computational challenges associated with large calcium imaging datasets and complex analyses are addressed by first improving the current workflow for greater efficiency and accessibility, and second, establishing a robust computaional workflow and data management strategy.
PI: Nick Guagliardo, Dept. of Pharmacology
Quantifying Calcium Oscillations
Calcium oscillations signify communication between zona glomerulosa cells of the mouse adrenal gland. Researchers in the Barrett Lab can capture these oscillatory events with calcium imaging, but they had difficulty analyzing the results. The Barrett Lab was in need of a comprehensive MATLAB program for quantitative analysis of the intracellular calcium signals from their cell imaging experiments. Prior to Research Computing’s involvement in the project, the Barrett Lab had been using fragments of code to analyze their data with little success. Research Computing developed a MATLAB application to create an efficient, centralized workflow that is also accessible to people who are new to MATLAB and programming.
